How To Calculate Absorbance From Percent Transmittance
How To Calculate Absorbance From Percent Transmittance . If all light passes through a solution without absorption, absorbance is zero, and percent transmittance is 100%. The absorbance has a logarithmic relationship to the transmittance with an absorbance of 0 corresponding to a transmittance of 100% and an absorbance of 1 corresponding to 10% transmittance. PPT Spectroscopy of Proteins PowerPoint Presentation ID6052251 from www.slideserve.com For example, a material transmitting 0.0001% would have an absorbance (optical density) of 6. In other words, it’s the amount of light that “successfully” passes through the substance and comes out the other side. Transmittance and absorbance have a logarithmic relationship.